Analysis of Chemical Composition by GC-MS and In-vitro Antidiabetic Activity of Azadirachta indica Leavesc Collected from Erbil, Iraq

Background: Azadirachta indica (Neem) is an important medicinal plant which is traditionally known for its
insecticidal and pharmacological properties.

Methods: The objective of this study was to evaluate in-vitro antidiabetic activity and characterize the chemical
constituents in ethyl acetate extract of the leaves of neem by using GC-MS. The in-vitro antidiabetic activity of neem
extract was evaluated via inhibition of α-amylase and α-glucosidase enzymes.

Results: GC-MS analysis of the ethyl acetate extract of neem resulted in the identification of 49 chemical compounds
and these constituents accounted to 97.07% of total composition. The major chemical components of the neem were
octadecanoic acid (7.17%), propyl tetracosyl ether (5.35%), 1-decanol, 2-octyl (5.01%), hexadecane (4.70%), tricosane
(4.73%), octadecane, 1-chloro (4.65%), and linolenic acid (4.35%). The neem extract produces dose dependent
inhibition of α-amylase and α-glucosidase enzymes. The IC₅₀ values for neem extract and acarbose 50 were 165.53±2.75
and 91.04±2.16 μg/mL, respectively against α-amylase enzyme. The IC₅₀ values for neem extract and acarbose were
210.08±2.16 and 120.05±2.16 μg/mL, respectively against α-glucosidase enzyme.

Conclusion: The study confirms the traditional claim of its use in the treatment of diabetes mellitus.