Preliminary Phytochemical and Pharmacological Screening of Aristolochia Bracteolata

Background and Aim: Aristolochia bracteolata Lam has been widely used in the traditional medicine for the treatment
of a variety of diseases. A. bracteolata belongs to the family Aristolochiaceae. It is easily available in all seasons. The
plant is mainly used in skin diseases, snake bite, arthritis and diabetes in Siddha system of medicine. In the present
study different extracts of roots and leaves of A. bracteolata were evaluated for their anti-inflammatory, antipruritic
and mast cell stabilizing activity, analgesic activity etc. The aim of the present study was to investigate total alkaloids
and flavonoids content of traditionally used medicinal plants of western region of India.

Method: The plants/plants parts were extracted by Soxhlet extraction methanol in methanol. Qualitative
phytohemical analysis was done for various phyto-constituents like alkaloids, tannins, cardiac glycosides, steroids and
saponins. Aqueous, methanol and chloroform extracts of this plant were evaluated for analgesic activity in Albino Rats.

Results: The methanolic extract of A. bracteolata contains alkaloids which were confirmed through TLC and HPTLC
analysis. The plant showed as a definite positive effect on analgesic activity with significant increase in the level of
powerful flavonoids. A bioassay-guided fractionation of methanol extract of A. bracteolata whole plant was used to
identify active compounds. The combined administration of the extract of plant and analgesic shows the more
analgesic activity in rats.

Conclusion: Preliminary phytochemical screening and pharmacological evaluations were analyzed by standard
methods. The results revealed that the methanolic extract showed significant analgesic activity.