Extraction, Biotransformation and In-vitro Biological Activity of Gymnema sylvestre Leaves Extracts and its Fermented Products

Background: Diabetes mellitus is a metabolic disorder which is characterized by an increase in blood glucose level. In
the Indian traditional system of medicine, various plant-based medicine are used to treat this disease; among them
Gymnema sylvestre is most commonly used. G. sylvestre plant extract contains various structurally related saponin
glycosides known as gymnemic acids, known for its antidiabetic potential. Fermented products are known to have
more therapeutic potential as compared to unfermented products, for the same purpose we have carried out
biotransformation of G. sylvestre plant extract.

Methods: Previously dried G. sylvestre leaves were grounded, and hydroalcoholic (70%) extraction was carried out
using soxhlet apparatus. Total crude extract, total saponin, gymnemic acid, and gymnemagenin content were analyzed
in hydroalcoholic plant extract, 5% w/v plant extract was biotransformed with Lactobacillus casei probiotic by
incubating plant extract with L. casei (1X10⁶ CFU) for 48h under anaerobic condition in MRS media, gymnemic acid, and
gymnemagenin contents were analyzed using toluene: chloroform: methanol (5:8:3, v/v/v) as a solvent system and
densitometric HPTLC scanning was performed at 610 nm after derivatization. Antioxidant potential, α-amylase, α-
glucosidase, and DPPH assay of G. sylvestre extract and its fermented products were carried out to check the
antidiabetic potential of plant extract and its fermented product.

Results: G. sylvestre leaves showed a yield of 32.35% w/w. The total saponin content was found to be 8.5% w/w of
leaves, and 25.68% w/w of the total plant extract. The biotransformation process significantly increases
gymnemagenin content from 1.21 to 3.34 μg/g. The antioxidant potential (IC₅₀ ) were found as 45.4 μg/mL, for G.sylvestre leaves extract and 38.4 μg/mL with its fermented product.

Conclusion: Thus, the present study gives an insight into the biotransformation of gymnemic acid to gymnemagenin
and its antidiabetic potential for the management of hyperglycemia.