Chromatographic Isolation and Spectroscopic Identification of Phytoconstituents of Ficus religiosa Linn. Stem Bark

Background: Ficus religiosa L. (Moraceae) known as Peepal, Bodhi and Ashwattha tree, is a native to India and southeastern
Asian countries. It is considered as a sacred tree in most of the part of south-eastern Asia. Traditionally it is used
to treat bacterial infections, asthma, bronchitis, chicken pox, constipation, cough, diabetes, epilepsy, gastric
problems, diarrhoea, leprosy, liver diseases, menstrual irregularities, migraine, sexual disorders, tuberculosis and
ulcers. The bark possesses aphrodisiac, anti-inflammatory, astringent, antiseptic and refrigerant properties, used to
treat anxiety, diabetes, boils, blisters, burns, diarrhoea, dysentery, gonorrhoea, inflammation, gout, piles, skin
diseases, urinogenital disorders, vomiting, wounds and to improve skin complexion. Ayurvedic formulations, such as
Chanda-nasavam, Nalpamaraditailam, Nyagrodhadi churna and Saribadyasavam contain F. religiosa bark as an
important ingredient. The aim of the study was the phytochemical investigation of the stem bark of F. religiosa.

Methods: The stem bark of F. religiosa (1 kg) was air dried and powdered. Extraction of coarsely powdered bark was
done exhaustively with methanol (2.5 L) using Soxhlet apparatus for 12 h. Isolation of phytoconstituents was
performed on glass column chromatography using silica gel. Precoated TLC plates were utilized for analysis of eluents
and the spots were visualized under UV lamp (254 and 366 nm). The structure of isolated phytoconstituents was
established on the basis of spectroscopic methods and chemical reactions.

Results: Phytochemical investigation of the methanol extract of F. religiosa stem bark led to the isolation of a new
naphthyl substituted phytosterol characterized as naphthyl-1ʹ,3ʹ-diol-1ʹ-3β-sitosteryl-3ʹ-linoleinate (1) and a new
lanostane type-triterpenic ester elucidated as lanostan-19-oic acid-3β-olyl-oleate (2) along with the known steroids
β-sitosteryl oleate (3) and β-sitosterol glucoside (4).

Conclusion: The present study has enhanced the knowledge base related to F. religiosa.. Isolated compounds (1) and
(2) are reported for the first time from this plant and may be considered as the markers for the quality control analysis
of marketed herbal formulations of F. religiosa.