Scolicidal Activity of Eriobotrya japonica and Lonicera japonica Extracts Against Hydatid Cysts of Echinococcus granulosus

Background and Objective: Nowadays, cystic echinococcosis is one of the parasitic zoonosis caused by Echinococcus. Surgery is the main treatment for this problem, but because of the possibility of spreading its content (protoscolices) during the surgery, finding a better way to prevent the production of secondary infection is always a remarkable subject. This study tried to investigate the Scolicidal effect of methanolic extract of Eriobotrya japonica and Lonicera japonica against hydatid cyst protoscolices in vitro. Materials and Methods: Three concentrations of Eriobotrya japonica and Lonicera japonica (25, 50, 75 mg mL^–1) were used over different times of exposure to 5, 10, 20 and 30 min. Then, the eosin stain method was used to determine the percentage of viability. Results: After 30 min of exposure to 75 μg mL^–1 of Eriobotrya japonica, the mean mortality rate was the highest amount (89.21%) than other samples. So Lonicera japonica was the most potent Scolicidal agent in the present study than Eriobotrya japonica. However, both extracts at the same time and concentration resulted in a higher mortality rate than the negative control group. Conclusion: As the results showed the Scolicidal effect of both extracts at three different concentrations was considerable and it has been increased over time. Based on these results, Lonicera japonica and Eriobotrya japonica can be considered candidates for the development of green Scolicidal.